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h dat  (Revvity)


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    Structured Review

    Revvity h dat
    H Dat, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+dat/10__3390_slash_molecules24203808-98-24-25?v=Revvity
    Average 91 stars, based on 19 article reviews
    h dat - by Bioz Stars, 2026-07
    91/100 stars

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    ( A, B ) Immunoprecipitation of DAT with two DAT antibodies directed against different DAT epitopes results in the co-precipitation of Gβ subunits from mouse striatum. Control experiments include immunoprecipitations with nonspecific rat or rabbit IgGs or immunoprecipitations from DAT knockout striatum or mouse cerebellum. ( C, D ) Immunoprecipitation of DAT resulted in the co-immunoprecipitation of Gβ subunits from MN9D-DAT cells or HEK293-DAT cells. Control experiments include immunoprecipitations from MN9D or HEK293 mock-transfected cells. Three different DAT antibodies were used; DAT 1 (Mab369, Millipore), <t>DAT</t> <t>2</t> (H-80, Santa Cruz), and DAT 3 (c-20, Santa Cruz). Immunoblotting was performed with a Gβ pan-antibody (T-20, Santa Cruz). Cereb = cerebellum, St = striatum, DAT-KO = DAT knockout.
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    ( A, B ) Immunoprecipitation of DAT with two DAT antibodies directed against different DAT epitopes results in the co-precipitation of Gβ subunits from mouse striatum. Control experiments include immunoprecipitations with nonspecific rat or rabbit IgGs or immunoprecipitations from DAT knockout striatum or mouse cerebellum. ( C, D ) Immunoprecipitation of DAT resulted in the co-immunoprecipitation of Gβ subunits from MN9D-DAT cells or HEK293-DAT cells. Control experiments include immunoprecipitations from MN9D or HEK293 mock-transfected cells. Three different DAT antibodies were used; DAT 1 (Mab369, Millipore), <t>DAT</t> <t>2</t> (H-80, Santa Cruz), and DAT 3 (c-20, Santa Cruz). Immunoblotting was performed with a Gβ pan-antibody (T-20, Santa Cruz). Cereb = cerebellum, St = striatum, DAT-KO = DAT knockout.
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    ( A, B ) Immunoprecipitation of DAT with two DAT antibodies directed against different DAT epitopes results in the co-precipitation of Gβ subunits from mouse striatum. Control experiments include immunoprecipitations with nonspecific rat or rabbit IgGs or immunoprecipitations from DAT knockout striatum or mouse cerebellum. ( C, D ) Immunoprecipitation of DAT resulted in the co-immunoprecipitation of Gβ subunits from MN9D-DAT cells or HEK293-DAT cells. Control experiments include immunoprecipitations from MN9D or HEK293 mock-transfected cells. Three different DAT antibodies were used; DAT 1 (Mab369, Millipore), DAT 2 (H-80, Santa Cruz), and DAT 3 (c-20, Santa Cruz). Immunoblotting was performed with a Gβ pan-antibody (T-20, Santa Cruz). Cereb = cerebellum, St = striatum, DAT-KO = DAT knockout.

    Journal: PLoS ONE

    Article Title: Inhibition of Dopamine Transporter Activity by G Protein βγ Subunits

    doi: 10.1371/journal.pone.0059788

    Figure Lengend Snippet: ( A, B ) Immunoprecipitation of DAT with two DAT antibodies directed against different DAT epitopes results in the co-precipitation of Gβ subunits from mouse striatum. Control experiments include immunoprecipitations with nonspecific rat or rabbit IgGs or immunoprecipitations from DAT knockout striatum or mouse cerebellum. ( C, D ) Immunoprecipitation of DAT resulted in the co-immunoprecipitation of Gβ subunits from MN9D-DAT cells or HEK293-DAT cells. Control experiments include immunoprecipitations from MN9D or HEK293 mock-transfected cells. Three different DAT antibodies were used; DAT 1 (Mab369, Millipore), DAT 2 (H-80, Santa Cruz), and DAT 3 (c-20, Santa Cruz). Immunoblotting was performed with a Gβ pan-antibody (T-20, Santa Cruz). Cereb = cerebellum, St = striatum, DAT-KO = DAT knockout.

    Article Snippet: Two different DAT antibodies were used for immunoprecipitations; DAT 1 (Mab369, Millipore), DAT 2 (H-80, Santa Cruz).

    Techniques: Immunoprecipitation, Knock-Out, Transfection, Western Blot